背根神经节
神经突
生物
降钙素基因相关肽
选择性拼接
RNA剪接
细胞生物学
分子生物学
寡核苷酸
信使核糖核酸
神经科学
基因亚型
作者
Lixia Tian,Yu Chen,Shuyang Chang,Linping Xu,Xiaoqiong Zhou,Qingxiang Mao,Lingli Liang
出处
期刊:Neuroreport
[Ovid Technologies (Wolters Kluwer)]
日期:2021-05-05
标识
DOI:10.1097/wnr.0000000000001625
摘要
Neuron-glial-related cell adhesion molecule (NrCAM) is a neuronal cell adhesion molecule that has been shown to be involved in several cellular processes in the peripheral nervous system, including neurite outgrowth. We recently reported that alternative splicing of Nrcam mRNA at exon 10 in the dorsal root ganglion (DRG) contributes to the peripheral mechanism of neuropathic pain. Specially, Nrcam antisense oligonucleotides (ASO) targeting Nrcam exon 10, attenuated neuropathic pain hypersensitivities in mice. Here, we investigated the effect of Nrcam ASO on neurite outgrowth of DRG neurons in vitro. By immunostaining DRG neurons with different DRG markers, Nrcam ASO significantly reduced neurite lengths in neurofilament 200-, calcitonin gene-related peptide and isolectin B4-positive neurons in primary DRG neuronal culture. Moreover, Nrcam ASO activates epidermal growth factor receptor, which may mediate the effect of Nrcam ASO on neurite outgrowth of cultured DRG neurons. These results provide evidence that Nrcam ASO suppresses neurite outgrowth in DRG neurons by regulating alternative splicing of Nrcam gene at exon 10 and activation of epidermal growth factor receptor signaling, indicating the differential roles of NrCAM variants/isoforms in neurite outgrowth.
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