聚己内酯
脚手架
材料科学
生物医学工程
椎间盘
体内
组织工程
静电纺丝
软骨
纳米纤维
抗压强度
PLGA公司
复合材料
生物相容性
纤维
离体
再生(生物学)
明胶
涂层
极限抗拉强度
生物材料
聚乙二醇
体外
化学
聚合物
解剖
医学
生物技术
有机化学
生物
作者
Morshed Khandaker,Hari Kotturi,Helga Progri,Subhakar Tummala,Sadegh Nikfarjam,P. Uday Chandra Rao,Asma Ul Hosna,Dhakshyane Tamil Arasu,Wendy R. Williams,Amgad M. Haleem
标识
DOI:10.1088/1748-605x/abfd12
摘要
Polyethylene glycol diacrylate (PEGDA) is an important class of photosensitive polymer with many tissue engineering applications. This study compared PEGDA and polycaprolactone (PCL) nanofiber matrix (NFM) coated PEGDA, referred to as PCL-PEGDA, scaffolds for their application in multiple tissue repair such as articular cartilage, nucleus pulposus of the intervertebral disc (IVD). We examined each scaffold morphology, porosity, swelling ratio, degradation, mechanical strength, andin vitrocytocompatibility properties. A defect was created in Sprague Dawley rat tail IVD by scraping native cartilage tissue and disc space, then implanting the scaffolds in the disc space for 4 weeks to evaluatein vivoefficacy of multi-tissue repair. Maintenance of disc height and creation of a new cell matrix was assessed to evaluate each scaffold's ability to repair the tissue defect. Although both PEGDA and PCL-PEGDA scaffolds showed similar porosity ∼73%, we observed distinct topographical characteristics and a higher effect of degradation on the water-absorbing capacity for PEGDA compared to PCL-PEGDA. Mechanical tests showed higher compressive strength and modulus of PCL-PEGDA compared to PEGDA.In vitrocell studies show that the PCL NFM layer covering PEGDA improved osteoblast cell adhesion, proliferation, and migration into the PEGDA layer.In vivostudies concluded that the PEGDA scaffold alone was not ideal for implantation in rat caudal disc space without PCL nanofiber coating due to low compressive strength and modulus.In vivoresults confirm that the PCL-PEGDA scaffold-maintained disc space and created a proteoglycan and collagen-rich new tissue matrix in the defect site after 4 weeks of scaffold implantation. We concluded that our developed PCL-PEGDA has the potential to be used in multi-tissue defect site repair.
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