3T3-L1 세포 모델에서 Adipogenesis 조절을 통한 녹색입 홍합 추출물의 항비만 활성

Adipogenesis was defined as a differentiation process of preadipocytes into adipocytes. It is a key process in determining the number of mature adipocytes in the development of obesity. Thus, to regulation of adipogenesis is crucial target in obesity prevention. Mussel (Mytilus corscus ) has been reported to possess abundant dietary proteins and savory amino acids. However, antiadipogenic role and function in obesity prevention remains unknown. Here, we employed green-shelled mussel and its water extract (GME) was prepared. To elucidate the potential role of GME in 3T3-L1 adipogenesis, 3T3-L1 cells were treated with GME between day –2 to day 6. We found that GME dose-dependently suppressed adipogenesis and inhibited formation of lipid droplets accompanied by reduced level of adipogenic transcriptional factors such as C/EBPα and FAS. Our study further revealed that inhibitory function of GME wa largely limited to the adipogenesis day 0 to day 6. Taken together, our evidences provide new insights into the molecular basis underlying the antiadipogenic function of GME.