脱氧核酶
粘液
金黄色葡萄球菌
化学
量油尺
DNA
劈理(地质)
微生物学
检出限
细菌
色谱法
生物化学
生物
遗传学
古生物学
尿
生态学
断裂(地质)
作者
M. Monsur Ali,Ryan Silva,Dawn White,Saeed Mohammadi,Yingfu Li,Alfredo Capretta,John D. Brennan
标识
DOI:10.1002/anie.202112346
摘要
Detection of pathogenic bacteria in complex biological matrices remains a major challenge. Herein, we report the selection and optimization of a new DNAzyme for Staphylococcus aureus (SA) and the use of the DNAzyme to develop a simple lateral flow device (LFD) for detection of SA in nasal mucus. The DNAzyme was generated by in vitro selection using a crude extra/intracellular mixture derived from SA, which could be used directly for simple solution or paper-based fluorescence assays for SA. The DNAzyme was further modified to produce a DNA cleavage fragment that acted as a bridging element to bind DNA-modified gold nanoparticles to the test line of a LFD, producing a simple colorimetric dipstick test. The LFD was evaluated with nasal mucus samples spiked with SA, and demonstrated that SA detection was possible in minutes with minimal sample processing.
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