Agrobacterium rhizogenes strain RPB13 induced highest survival percentage (100%) along with the highest no. of roots per explant, whereas, in hormone treated micro-propagated explants the survival percentage was 94%.The maximum number. of roots were 11 and 6 due to the treatment with A. rhizogenes and hormones respectively per explant. Furthermore, the induction of rooting in explant by strain RPB13 required less days than that of hormonal treatment. Thus, A. rhizogenes strain RPB13 has opened new avenue for rooting in micro-propagated explants without hormonal use in strawberry plants.