类胡萝卜素
重组DNA
抗菌剂
生物
抗体
血液蛋白质类
生物化学
化学
微生物学
抗菌肽
分子生物学
免疫学
基因
作者
Ole E. Sørensen,Jack B. Cowland,Jon Askaa,Niels Borregaard
标识
DOI:10.1016/s0022-1759(97)00084-7
摘要
hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 μg protein per 106 cells. The plasma level is 1.18 μg/ml which is several fold higher than for other neutrophil specific granule proteins. hCAP-18 is present in plasma as high molecular weight complexes. In accordance with this, hCAP-18 is barely excreted in the urine. The bone marrow appears to be the major source of plasma hCAP-18. The high level of hCAP-18 in plasma may provide an important defense against microorganisms and endotoxins.
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