Experimental Diabetes Upregulates the Expression of Uretereral Endothelin Receptors

NAKAMURA, I., M. SAITO, Y. FUKUMOTO, M. YOSHIDA, K. NISHI, R. M. WEISS AND J. LATIFPOUR. Experimental diabetes upregulates the expression of uretereral endothelin receptors. PEPTIDES 18(7) 1091–1093, 1997.—We investigated the binding characteristics of endothelin (ET) receptors in the ureters of rats with experimentally induced diabetes and diuresis. Receptor binding experiments demonstrated an upregulation in the expression of [125I]ET-1 binding sites in the diabetic rat ureter but not in the diuretic rat ureter. ET-1, ET-3, IRL 1620, and BQ 610 inhibited [125I]ET-binding to the rat ureter consistent with the predominance of ETA receptors in these tissues. The subtype specificity of ET receptors in ureteral tissues was confirmed with inhibition data obtained from cloned human ETA and ETB receptors.