化学
乳酸菌
核磁共振波谱
二维核磁共振波谱
多糖
单体
核磁共振谱数据库
立体化学
发酵
聚合物
有机化学
谱线
物理
天文
作者
Lindsay P. Harding,Valerie M. Marshall,Mark Elvin,Yu‐Cheng Gu,Andrew P. Laws
标识
DOI:10.1016/s0008-6215(02)00354-3
摘要
The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:Download : Download full-size imageA process for characterising polysaccharides having low solubility in aqueous solution is reported.
科研通智能强力驱动
Strongly Powered by AbleSci AI