压电1
基因敲除
背根神经节
机械敏感通道
RNA干扰
离子通道
细胞生物学
跨膜蛋白
生物
基因
背
遗传学
核糖核酸
解剖
受体
作者
Bertrand Coste,Jayanti Mathur,Manuela Schmidt,Taryn J. Earley,Sanjeev S. Ranade,Matt Petrus,Adrienne E. Dubin,Ardem Patapoutian
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2010-10-01
卷期号:330 (6000): 55-60
被引量:2067
标识
DOI:10.1126/science.1193270
摘要
Mechanical stimuli drive many physiological processes, including touch and pain sensation, hearing, and blood pressure regulation. Mechanically activated (MA) cation channel activities have been recorded in many cells, but the responsible molecules have not been identified. We characterized a rapidly adapting MA current in a mouse neuroblastoma cell line. Expression profiling and RNA interference knockdown of candidate genes identified Piezo1 (Fam38A) to be required for MA currents in these cells. Piezo1 and related Piezo2 (Fam38B) are vertebrate multipass transmembrane proteins with homologs in invertebrates, plants, and protozoa. Overexpression of mouse Piezo1 or Piezo2 induced two kinetically distinct MA currents. Piezos are expressed in several tissues, and knockdown of Piezo2 in dorsal root ganglia neurons specifically reduced rapidly adapting MA currents. We propose that Piezos are components of MA cation channels.
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