体内
间充质干细胞
壳聚糖
化学
体外
脚手架
生物医学工程
生物物理学
组织工程
材料科学
细胞生物学
生物化学
生物
医学
生物技术
作者
Zhi Xiong Huang,Bo Yu,Qingling Feng,Songjian Li,Yan Chen,Lingaï Luo
标识
DOI:10.1016/j.carbpol.2011.02.029
摘要
A biomimetic and thermosensitive gel scaffold was prepared from chitosan (CS), nano-hydroxyapatite (HA) and collagen (Col). The changes of storage modulus, loss modulus, pH and conductivity of the CS/HA/Col system as a function of temperature confirmed the liquid-to-gel phase transition process occurred under physiological conditions. The formed gel acted as a biocompatible substrate for the proliferation of rat bone marrow stem cells (rBMSCs) in vitro. rBMSCs suspension was co-injected with CS/HA/Col solution into Wistar rats. The injected rBMSCs survived in the CS/HA/Col gel for 28 days in vivo. The CS/HA/Col/rBMSCs gels induced less inflammatory reaction in the host tissue than the CS/HA/Col gels. Our results suggest that the CS/HA/Col system can be used to load rBMSCs in vitro homogeneously. The CS/HA/Col system can be injected into body in a minimally invasive manner and provides a biocompatible environment for rBMSCs survival in vivo.
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