胚状病毒
开放式参考框架
生物
基因组
粉虱
双子病毒科
病毒学
基因组组织
卷叶
全基因组测序
植物病毒
核酸序列
病毒
遗传学
DNA
基因
植物
打开阅读框
肽序列
作者
Qinyan Yin,Hongzhou Yang,Qianhong Gong,Huanyu Wang,Yule Liu,Yiguo Hong,Po Tien
出处
期刊:Virus Research
[Elsevier]
日期:2001-12-01
卷期号:81 (1-2): 69-76
被引量:36
标识
DOI:10.1016/s0168-1702(01)00363-x
摘要
The complete DNA sequence (2734 nucleotides) of the monopartite genome of tomato yellow leaf curl China virus (TYLCCNV), a begomovirus transmitted by the whitefly Bemisia tabaci, was determined. The circular genomic DNA contains six open reading frames (ORFs) encoding proteins of molecular weights >10 kDa, of which two (V1 and V2) are located on the virion-sense strand and four (C1, C2, C3 and C4) on the complementary-sense strand. The ORFs are comparable to those of other whitefly-transmitted begomoviruses with a monopartite genome and to those encoded by DNA-A of bipartite begomoviruses. Sequence comparisons with other geminiviruses showed that TYLCCNV belongs to Begomovirus from the Old World. No putative DNA-B genome was found. Nicotiana species and tomato plants agroinoculated with the TYLCCNV monopartite genome developed typical yellowing and leaf-curling symptoms. The cloned molecule carried all the information needed for virus replication and systemic infection of plants.
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