血小板源性生长因子受体
山奈酚
磷酸化
血管平滑肌
信号转导
酪氨酸磷酸化
血小板衍生生长因子
细胞生物学
生物
蛋白激酶B
细胞内
细胞生长
分子生物学
受体
内分泌学
生物化学
生长因子
类黄酮
平滑肌
抗氧化剂
作者
Soo-Yeon Kim,Yong-Ri Jin,Yong Lim,Jin-Ho Kim,Michael Cho,Hong Jiang,Hwan‐Soo Yoo,Young Won Yun
出处
期刊:Planta Medica
[Georg Thieme Verlag KG]
日期:2005-07-01
卷期号:71 (7): 599-603
被引量:19
标识
DOI:10.1055/s-2005-871263
摘要
Kaempferol, a flavonoid present in human diet and plants, has been known to show cardiovascular protection via its anti-oxidant activity. In this study, we have investigated the effect of kaempferol on the proliferation of primary cultured rat aortic vascular smooth muscle cells (VSMCs). Kaempferol significantly inhibited 50 ng/mL platelet-derived growth factor (PDGF)-BB-induced proliferation and [3 H]-thymidine incorporation into DNA at concentrations of 5, 20 and 50 µM without any cytotoxicity. Kaempferol also inhibited the c-fos mRNA expression induced by PDGF-BB concentration-dependently. In addition, consistent with the inhibition of cell proliferation and c-fos mRNA expression, kaempferol inhibited the PDGF beta-receptor (Rβ) phosphorylation in a concentration-dependent manner. Accordingly, the downstream signal transductions of PDGF-Rβ such as ERK1/2, Akt and PLC-γ1 phosphorylation were also inhibited by kaempferol in the same pattern. These findings suggest that, in addition to its anti-oxidant activity, the cardiovascular protective effect of kaempferol may be mediated, at least in part, by the suppression of VSMC proliferation, which is due to the inhibition of PDGF-Rβ tyrosine phosphorylation and its downstream intracellular signal transduction.
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