O55 | Risk of residues of toltrazuril sulfone in eggs after oral administration

ABSTR ACTDust in animal production contains particles of feces, organic materials, and antimicrobial residues, which remain in suspension, and are disseminated through the environment by air currents or individual's movement.Sulfachloropyridazine (SCP) is an antimicrobial of the sulfonamide family, which is widely used in the poultry industry.For this reason, the aim of the study was to evaluate the environmental dissemination of SCP via droppings from treated to untreated chickens.Materials and Methods: Three experimental groups were available (A, B.1 and B.2) and 1 control group (C) of 10 Broiler chickens each, bred under controlled conditions.Group A was treated with a commercial formulation of SCP for 5 days, while the other two groups were not treated and were in an adjacent pen (B.1) and in a pen 30 cm away (B.2) from the treated group.Subsequently, dropping samples were taken from all groups by cloacal stimulation on days 3, 6, 9, 12, 15 and 18 after the treatment, for the SCP analysis by HPLC-MS/MS. Results:In group A, residual SCP concentrations were found up to day 15 post-treatment, which ranged from 18.62 to 718.17 μg/kg on average.In group B.1 and group B.2 SCP residues were detected in concentrations below the Limit of Quantification, which lasted until day 12 post-treatment and day 3 post-treatment, respectively.Statistical difference between the study groups was determined according to a one-factor ANOVA on the SCP concentrations from the 3 groups (p-value < .05).Subsequently, a one-factor ANOVA was performed on the SCP concentrations from groups B.1 and B.2, with a p-value > .05,establishing that there is no significant difference in the dissemination at the different distances applied. Conclusions:There is a degree of SCP dissemination in the production environment from the droppings of a group of broilers treated with SCP, so that the droppings of treated productive animals may be a source of antimicrobial residue contamination.