化学
葡聚糖
神经保护
色谱法
高效液相色谱法
乙醇
天然产物
立体化学
有机化学
药理学
生物
酶
作者
Xiao Yu,Lin-Lin Zhu,Chang Xu,Su Chen,Yan Liang,Bin Liu,Wei Zhang
出处
期刊:PubMed
日期:2022-09-01
卷期号:47 (17): 4650-4657
标识
DOI:10.19540/j.cnki.cjcmm.20220419.201
摘要
The present study investigated the chemical constituents from Uncaria sessilifructus and their neuroprotective activities. The compounds were separated and purified from the 90% ethanol extract of U. sessilifructus by various chromatographic methods, including silica gel, Sephadex LH-20, and semi-preparative HPLC. Seven compounds were obtained, and their structures were identified as uncanidine J(1), uncanidine K(2), 17-O-ethylhirsutine(3), tetrahydroalstonine(4), akuammigine(5), hirsutine(6), and hirsuteine(7) by physicochemical properties and various spectral techniques, including UV, IR, MS, and NMR. Compounds 1 and 2 are two new compounds. Compound 3 is a new natural product, and compound 4 was isolated from U. sessilifructus for the first time. In addition, the isolated compounds were evaluated for their neuroprotective effects on oxygen and glucose deprivation/reoxygenation(OGD/R) injury in primary cortical neurons in rats. The results showed that compounds 1-7 had different degrees of protective effects on OGD/R injury. The EC_(50) values of compounds 2-4 were(0.17±0.03),(1.70±0.38), and(1.79±0.23) μmol·L~(-1), respectively.
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