Asymmetric reflection quantitative phase microscopy with scalable Field-of-View using dynamic speckle illumination

Dynamic speckle illumination-based quantitative phase microscopy (QPM) offers the capability to eliminate coherent noise and achieve depth selection; however, the low coherence of the illumination restricts the flexibility in objective lens selection. An asymmetric reflective quantitative phase microscopy method is proposed in this Letter. The speckle field correlation is maintained through identical exit pupil diameters in the objectives of both interference arms. Moreover, a light source system with a delay line compensates for the optical path difference introduced by the asymmetric objectives, thereby achieving a high-contrast interferogram. Experimental measurements on a resolution target and a transparent sample demonstrate the dynamic phase imaging and depth-selection capabilities of the system across different fields of view.