Aging amplifies sex differences in low alpha and low beta EEG oscillations

Biological sex profoundly shapes brain function, yet its precise influence on neural oscillations was poorly understood. Despite decades of research, studies investigating sex-based variations in electroencephalographic (EEG) signals have yielded inconsistent findings that obstructs what may be a potentially crucial source of inter-individual variability in brain function. To address this, we analyzed five publicly available resting-state datasets, comprising EEG data (n = 445) and iEEG data (n = 103). Three age ranges were defined, young adult (YA, 18-30 years), middle-aged adult (MA, 30-55 years) and older adult (OA, 55-80 years). Our results revealed striking age-dependent sex differences: OA group exhibited robust sex differences, with males showing heightened low alpha (8-9 Hz) activity in temporal regions and attenuated low beta (16-20 Hz) oscillations in parietal-occipital areas compared to females. Intriguingly, these sex-specific patterns were absent in YA group, suggesting a complex interplay between sex and aging in shaping brain dynamics. The MA groups fall in between YA and OA group. The increase of low beta band activity in older female adults is strongly associated with hip size and BMI. Furthermore, we identified consistent sex-related activity in the precentral gyrus with the results of scalp EEG, potentially driving the observed scalp EEG differences. This multi-level analysis allowed us to bridge the gap between cortical and scalp-level observations, providing a more comprehensive picture of sex-related neural dynamics. The distinct associations between sex-specific oscillatory patterns and several lifestyle factors demonstrates the complex interplay between sex, age, and neural oscillations, revealing the variability in brain dynamics. Our findings highlight the importance of careful demographic consideration in EEG research design to ensure fairness in capturing the full spectrum of neurophysiological diversity.