Antibacterial polylactic acid fabricated via Pickering emulsion approach with polyethyleneimine and polydopamine modified cellulose nanocrystals as emulsion stabilizers

Antibacterial biodegradable plastics are highly demanded for food package and disposable medical plastic consumables. Incorporating antibacterial nanoagents into polymer matrices is an effective method to endow polymers with antibacterial activity. However, synthesis of sustainable antibacterial nanoagents with high antibacterial activity via facile approach and well dispersion of them in polymer matrices are still challenging. In this study, polyethyleneimine (PEI) was grafted on surface of cellulose nanocrystals (CNCs) via the oxidation self-polymerization of dopamine (DA) and the Michael addition/Schiff base reaction between DA and PEI. The resulted PEI and polydopamine modified CNCs (PPCs) showed substantially enhanced antibacterial activity and reduced cytotoxicity for NIH3T3 than PEI due to increased local concentration and anchoring of PEI. The minimum concentration of PPCs to achieve antibacterial rate of 99.99 % against S. aureus and E. coli were about 50 and 20 μg/mL, respectively. PPCs displayed outstanding emulsifying ability, and PPC coated polylactic acid (PLA) microspheres were obtained by drying PPC stabilized PLA Pickering emulsion, leading to a well dispersion of PPCs in PLA. PPC/PLA film prepared by hot-pressing displayed great antibacterial performance and enhanced mechanical properties. Therefore, this study proposed a facile approach to fabricate biocompatible antibacterial nanoagents and plastics.