等位基因
单核苷酸多态性
聚合酶链反应
生物
SNP公司
步伐
遗传学
PCR变异
分子生物学
基因
基因型
地理
大地测量学
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 263-271
被引量:2
标识
DOI:10.1007/978-1-0716-3024-2_18
摘要
PCR allele competitive extension (PACE™) is a marker system to analyze single nucleotide polymorphisms (SNPs). As strongly related technologies like kompetitive allele-specific PCR (KASP™) or Amplifluor®, PACE is based on a polymerase chain reaction (PCR) with two competing allele-specific primers and an endpoint fluorescent measurement. The system requires sequence information surrounding the SNP of interest and a qPCR machine or a fluorescent plate reader.
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