诱导多能干细胞
细胞生物学
MAPK/ERK通路
生物
转录因子
pou结构域
PI3K/AKT/mTOR通路
激酶
干细胞
蛋白激酶B
细胞分化
信号转导
胚胎干细胞
遗传学
基因
同源盒
作者
Xinchun Yang,Xiaolong Wu,Wenhao Li,Xue-Ru Wu,Qian-Yan Shen,Yunxiang Li,Sha Peng,Jinlian Hua
标识
DOI:10.24272/j.issn.2095-8137.2022.220
摘要
As a transcription factor of the Pit-Oct-Unc (POU) domain family, octamer-binding transcription factor 6 (OCT6) participates in various aspects of stem cell development and differentiation. At present, however, its role in porcine-induced pluripotent stem cells (piPSCs) remains unclear. Here, we explored the function of OCT6 in piPSCs. We found that piPSCs overexpressing OCT6 maintained colony morphology and pluripotency under differentiation conditions, with a similar gene expression pattern to that of non-differentiated piPSCs. Functional analysis revealed that OCT6 attenuated the adverse effects of extracellular signal-regulated kinase (ERK) signaling pathway inhibition on piPSC pluripotency by activating phosphatidylinositol 3-kinase-protein kinase B (PI3K-AKT) signaling activity. Our research sheds new light on the mechanism by which OCT6 promotes PSC maintenance.
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