微泡
血管生成
脐静脉
细胞生物学
小胶质细胞
新生血管
趋化因子
视网膜
化学
分子生物学
体外
癌症研究
免疫学
炎症
生物
小RNA
生物化学
基因
神经科学
作者
Yan Wang,J. Wang,Ting Pan,Jie Liu,Fan Yang,Jingfan Wang,Xu Chen,Qinyuan Gu,Xingxing Wang,Tianlin Xiao,Ping Xie,Ping Xie,Zizhong Hu
标识
DOI:10.1016/j.exer.2024.109837
摘要
The lens is an avascular tissue, where epithelial cells (LECs) are the primary living cells. The role of LECs-derived exosomes (LEC-exos) is largely unknown. In our study, we determined the anti-angiogenic role of LEC-exos, manifested as regressed retinal neovascularization (NV) using the oxygen-induced retinopathy (OIR), and reduced choroidal NV size and pathological vascular leakage using the laser-induced choroidal neovascularization (laser-induced CNV). Furthermore, the activation and accumulation of microglia were also restricted by LEC-exos. Based on Luminex multiplex assays, the expressions of chemokines such as SCYB16/CXCL16, MCP-1/CCL2, I-TAC/CXCL11, and MIP 3beta/CCL19 were decreased after treatment with LEC-exos. Transwell assays showed that LEC-exos restricted the migration of the mouse microglia cell line (BV2 cells). After incubation with LEC-exos-treated BV2 cells, human umbilical vein endothelial cells (hUVECs) were collected for further evaluation using tube formation, Transwell assays, and 5-ethynyl-2′-deoxyuridine (EDU) assays. Using in vitro experiments, the pro-angiogenic effect of microglia was restricted by LEC-exos. Hence, it was investigated that LEC-exos attenuated ocular NV, which might attribute to the inhibition of microglial activation and accumulation.
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