鲍曼不动杆菌
平移(音频)
噬菌体展示
微生物学
生物
计算生物学
单克隆抗体
细菌
抗体
免疫学
遗传学
铜绿假单胞菌
古生物学
缩放
镜头(地质)
作者
Gregory A. Knauf,Kyra E. Groover,Angela C. O’Donnell,Bryan William Davies
出处
期刊:ACS Infectious Diseases
[American Chemical Society]
日期:2023-05-10
卷期号:9 (6): 1190-1195
被引量:2
标识
DOI:10.1021/acsinfecdis.3c00024
摘要
The bacterial pathogen Acinetobacter baumannii is a leading cause of drug-resistant infections. Here, we investigated the potential of developing nanobodies that can recognize A. baumannii over other Gram-negative bacteria. Through generation and panning of a synthetic nanobody library, we identified several potential lead candidates. We demonstrate how incorporation of next-generation sequencing analysis can aid in the selection of lead candidate nanobodies. Using monoclonal phage display, we validated the binding of lead nanobodies to A. baumannii. Subsequent purification and biochemical characterization revealed one particularly robust nanobody that specifically bound select A. baumannii strains compared to other common drug-resistant pathogens. These findings support the potential for nanobodies to selectively target A. baumannii and the identification of lead candidates for future investigation.
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