[Expression and Clinical Significance of Exosome Derived MiR-181b-5p in Children with Acute Lymphoblastic Leukemia].

To explore the expression level of exosome derived miR-181b-5p in different disease stages of children with acute lymphoblastic leukemia and its relationship with clinical characteristics.Bone marrow plasma samples of 86 children with ALL were collected. Exosomes were extracted by exosome extraction kit, and RNA in exosomes was extracted by TRIzol method. The levels of miR-181b-5p in the blood plasma exosomes of the patients in the newly diagnosed group, relapse group, remission group and control group were detected by qRT- PCR. The difference of miR-181b-5p expression level in each group was compared and analyzed, and the relationship between miR-181b-5p expression level and clinical characteristics was analyzed.The expression level of exosomal miR-181b-5p in the newly diagnosed group and the relapsed group was significantly lower than that in the remission group and the control group (P< 0.05). The expression level of exosomal miR-181b-5p in T-ALL children was higher than that in B-ALL children (P<0.05). The expression level of plasma exosomal miR-181b-5p in male children was higher than that in female children (P<0.01).Exosome derived miR-181b-5p changes dynamically in the course of ALL children, and can be used as a marker miRNA to monitor disease status. Exosomes can transmit information in the tumor microenvironment and serve as a potential carrier for biomolecular targeted therapy.外泌体源性miR-181b-5p在儿童急性淋巴细胞白血病中的表达及临床意义.探讨外泌体源性miR-181b-5p在急性淋巴细胞白血病患儿不同疾病阶段的表达水平差异及其与临床特点的相关性.收集86例ALL患儿骨髓血浆标本，采用外泌体提取试剂盒提取血浆中外泌体，TRIzol法提取外泌体中RNA，采用实时荧光定量聚合酶链式反应(qRT-PCR)法检测初诊组、复发组、缓解组、对照组患儿血浆外泌体miR-181b-5p表达水平，比较分析miR-181b-5p表达水平在各组患儿中的差异，以及miR-181b-5p表达水平与临床特征之间的关系.初诊和复发组ALL患儿血浆外泌体miR-181b-5p表达水平均明显低于缓解组和对照组（P<0.05）；初诊组T-ALL患儿血浆外泌体miR-181b-5p表达水平明显高于B-ALL患儿（P<0.05）；初诊组男性患儿血浆外泌体miR-181b-5p表达水平明显高于女性患儿（P<0.01）.外泌体源性miR-181b-5p在ALL儿童病程中呈动态变化，可以作为监测疾病状态的标记性miRNA；外泌体可以在肿瘤微环境中传递信息，作为生物分子靶向治疗的潜在载体.