全身成像
临床前影像学
帧速率
生物医学工程
计算机科学
体内
核医学
计算机视觉
医学
生物
正电子发射断层摄影术
生物技术
作者
Lei Li,Linyu Zhu,Cheng Ma,Li Lin,Junjie Yao,Lidai Wang,Konstantin Maslov,Ruiying Zhang,Wanyi Chen,Junhui Shi,Lihong V. Wang
标识
DOI:10.1038/s41551-017-0071
摘要
Imaging of small animals has played an indispensable role in preclinical research by providing high-dimensional physiological, pathological and phenotypic insights with clinical relevance. Yet, pure optical imaging suffers from either shallow penetration (up to ~1–2 mm) or a poor depth-to-resolution ratio (~3), and non-optical techniques for whole-body imaging of small animals lack either spatiotemporal resolution or functional contrast. Here, we demonstrate that stand-alone single-impulse panoramic photoacoustic computed tomography (SIP-PACT) mitigates these limitations by combining high spatiotemporal resolution (125 μm in-plane resolution, 50 μs per frame data acquisition and 50 Hz frame rate), deep penetration (48 mm cross-sectional width in vivo), anatomical, dynamical and functional contrasts, and full-view fidelity. Using SIP-PACT, we imaged in vivo whole-body dynamics of small animals in real time and obtained clear sub-organ anatomical and functional details. We tracked unlabelled circulating melanoma cells and imaged the vasculature and functional connectivity of whole rat brains. SIP-PACT holds great potential for both preclinical imaging and clinical translation. Single-impulse photoacoustic computed tomography can, at deep penetration and high resolution and contrast, image the whole-body dynamics of small animals in real time, and track injected cancer cells and image the vasculature of whole rat brains.
科研通智能强力驱动
Strongly Powered by AbleSci AI