生物传感器
检出限
纳米复合材料
电化学
钝化
核化学
DNA
材料科学
纳米技术
电极
化学
组合化学
色谱法
生物化学
物理化学
图层(电子)
作者
Qitong Huang,Xiaofeng Lin,Jie-Ji Zhu,Qing‐Xiao Tong
标识
DOI:10.1016/j.bios.2017.03.048
摘要
In this study, a green and fast method was developed to synthesize high-yield carbon dots (CDs) via one-pot microwave treatment of banana peels without using any other surface passivation agents. Then the as-prepared CDs was used as the reducing agent and stabilizer to synthesize a [email protected] nanocomposite by a simple sequential reduction strategy. Finally, [email protected] nanocomposite modified glassy carbon electrode ([email protected]/GCE) was obtained as a biosensor for target DNA after being immobilized a single-stranded probe DNA by a carboxyl ammonia condensation reaction. Under the optimal conditions, the sensor could detect target DNA concentrations in the range from 5.0×10–16 to 1.0×10−1° mol L−1. The detection limit (LD) was estimated to be 1.82×10–17 mol L−1, which showed higher sensitivity than other electrochemical biosensors reported. In addition, the DNA sensor was also successfully applied to detect colitoxin DNA in human serum.
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