Состав и содержание флавоноидов видов растений из секций Eudmus Bunge и Melilotopsis Gontsch. Рода Astragalus l. Сибири

Using HPLC method, composition and content of the flavonoid aglycons and glycosides were studied in the leaves of species from section Eudmus (Astragalus uliginosus L., A. schelichovii Turcz.) and section Melilotopsis Gontsch. (A. melilotoides Pall., A. tenuis Turcz.) of the genus Аstragalus L. from different habitats. In extracts' hydrolysates of A. uliginosus and A. schelichovii leaves, five flavonoid aglycones have been found. Quercetin, kaempferol and isorhamnetin have been identified in them. Morphological and geographical differentiation of these species is confirmed by the taxon specific composition of the flavonoid glycosides. The average value of the sum of major flavonoids glycosides has been as follows: in leaves of A. uliginosus plants 2.46 % (by weight of the absolutely dry raw material), in A. schelichovii 3.08 %. In A. melilotoides, quercetin, kaempferol, isorhamnetin, rutin and isoquercitrin have been found, and in A. tenuis, there have been quercetin, kaempferol, isorhamnetin, luteolin, isoquercitrin, quercetin-3-O-p-glucobioside. Multicomponent composition of phenolic compounds of the A. melilotoides (33 substances) distinguishes it from composition of the A. tenuis (12 substances). The content of phenolic compounds in A. melilotoides amounted to 6.0 %, A. tenuis to 7.0 %, including quercetin-3-O-p-glucobioside to 4.30 %. Flavonoid composition and content of the species of Eudmus and Melilotopsis sections can serve as chemotaxonomic markers in the study of systematic classification of plants. The high content of substances allows us to recommend the species investigated as sources of flavonoids.