去细胞化
软骨
软骨发生
细胞外基质
甲基丙烯酸缩水甘油酯
材料科学
再生(生物学)
间充质干细胞
关节软骨修复
生物医学工程
纳米纤维
细胞生物学
化学
复合材料
聚合
解剖
关节软骨
骨关节炎
聚合物
病理
生物化学
生物
医学
替代医学
作者
Hye Sung Kim,Nandin Mandakhbayar,Hae‐Won Kim,Kam W. Leong,Hyuk Sang Yoo
出处
期刊:Biomaterials
[Elsevier]
日期:2021-02-01
卷期号:269: 120214-120214
被引量:50
标识
DOI:10.1016/j.biomaterials.2020.120214
摘要
Cartilage defect is difficult to heal due to its avascular properties. Implantation of mesenchymal stem cell is one of the most promising approach for regenerating cartilage defects. Here we prepared polymeric nanofibrils decorated with cartilage-derived decellularized extracellular matrix (dECM) as a chondroinductive scaffold material for cartilage repair. To fabricate nanofibrils, eletrospun PCL nanofibers were fragmented by subsequent mechanical and chemical process. The nanofibrils were surface-modified with poly(glycidyl methacrylate) ([email protected]) via surface-initiated atom transfer radical polymerization (SI-ATRP). The epoxy groups of [email protected] were subsequently reacted with dECM prepared from bovine articular cartilage. Therefore, the cartilage-dECM-decorated nanofibrils structurally and biochemically mimic cartilage-specific microenvironment. Once adipose-derived stem cells (ADSCs) were self-assembled with the cartilage-dECM-decorated nanofibrils by cell-directed association, they exhibited differentiation hallmarks of chondrogenesis without additional biologic additives. ADSCs in the nanofibril composites significantly increased expression of chondrogenic gene markers in comparison to those in pellet culture. Furthermore, ADSC-laden nanofibril composites filled the osteochondral defects compactly due to their clay-like texture. Thus, the ADSC-laden nanofibril composites supported the long-term regeneration of 12 weeks without matrix loss during joint movement. The defects treated with the ADSC-laden [email protected] significantly facilitated reconstruction of their cartilage and subchondral bone ECM matrices compared to those with ADSC-laden nanofibrils, non-specifically adsorbing cartilage-dECM without surface decoration of PGMA.
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