[Detection and Analysis of T Lymphocyte Subsets and B Lymphocytes in Patients with Acute Leukemia].

To investigate the changes of T lymphocyte subsets, B lymphocyte and NK cells in peripheral blood of patients with acute leukemia at different periods and their significance.The peripheral T lymphocyte subsets and B lymphocyte of 95 patients with acute leukemia [(43 cases of acute lymphoblastic leukemia (ALL), 52 cases of acute myeloid leukemia (AML)] and 50 normal people were detected by flow cytometry respectively.The positive rate of CD3+, CD3+CD4+, CD3+CD8+, NK cells and CD4+/CD8+ in the patients with newly diagnosed acute leukemia were significantly lower than those in normal controls (P<0.05) , but increased obviously after complete remission. The positive rate of Treg cells in the patients with newly diagnosed leukemia group was significantly higher than that in normal controls (P<0.01) , but decreased obviously after complete remission. Positive rate of CD3-CD19+ cells in the patients with newly diagnosed acute myeloid leukemia was significantly lower , but higher in the patients with newly diagnosed acute lymphoblastic leukemia than that in normal controls with statistical significant difference (P<0.05) , and increased obviously in the patients with acute myeloid leukemia (P<0.05) after complete remission , but decreased in the patients with acute lymphoblastic leukemia (P<0.01) after complete remission or no-remission.Changes of T lymphocyte subsets, B lymphocytes and NK cells in the patients with newly diagnosed acute leukemia are significant, thus the detection of T lymphocyte cell subsets, B lymphocytes and NK cells can provide some evidences. for evaluation of the disease severity, curative efficiency and prognosis of patients with acute leukemia.急性白血病患者T、B淋巴细胞的检测与分析.探讨不同类型的急性白血病患者外周血T淋巴细胞亚群、B淋巴细胞和NK细胞等在疾病的不同时期的变化及其临床意义。.采用流式细胞术检测95例初诊白血病患者[急性淋巴细胞白血病（ALL）43例，急性髓系白血病（AML）52例]诱导治疗前后及50例正常对照者外周血T淋巴细胞亚群、B淋巴细胞、NK细胞及Treg细胞百分比。.初诊白血病各组CD3+、CD3+CD4+、CD3+CD8+、NK细胞阳性率和CD4+/CD8+比值均明显低于正常对照组（P＜0.05），治疗后完全缓解患者的上述指标均明显高于治疗前（P＜0.05）。初诊白血病各组患者Treg细胞阳性率均高于正常对照组（P＜0.01），AML和ALL治疗组完全缓解患者Treg细胞阳性率较治疗前有所降低（P＜0.05）。AML初诊组CD3-CD19+细胞阳性率明显低于正常对照组（P＜0.01），ALL初诊组CD3-CD19+细胞阳性率高于正常对照组（P＜0.01）；治疗后AML组缓解患者CD3-CD19+细胞阳性率高于治疗前（P＜0.05），ALL组缓解患者CD3-CD19+细胞阳性率明显低于治疗前（P＜0.01）；ALL治疗后未缓解者CD3-CD19+细胞阳性率明显低于治疗前（P＜0.01）；ALL治疗后未缓解者的其余各项指标及AML治疗后未缓解者各项指标与治疗前比较无统计学差异。.初诊白血病各组T淋巴细胞亚群、B淋巴细胞和NK细胞的变化明显，完全缓解组的上述指标基本恢复正常，说明T淋巴细胞亚群、B淋巴细胞、NK细胞水平检测在评价急性白血病严重程度、疗效及判断患者预后有一定的临床价值。.