The optimum conditions for selective .elimination of salt from tuna boiled extract (TBE) by electrodialysis were determined. The desalination conditions of TBE were determined at various pH's, concentrations and volumes of TBE. The ion-exchange membrane with a molecular weight cut off 100Da was used for desalting of TBE. The desalination times on and of TBE concentrations were 40 min and 240 min, respectively. The electrodialysis process could removed above of the initial salt content in and of TBE concentrations. The desalination of TBE at pH 4.0 was higher than that at pH 9.0 The amount of water transferred by the electrodialysis was determined. The electrodialysis process could remove above of the initial salt content in TBE for 80 min. The initial volume and the permeate did not have significant effects on desalination time and ratio. The key parameters for the desalination of TBE were pH and concentration of TBA.