Activated Platelets Release Two Types of Membrane Vesicles: Microvesicles by Surface Shedding and Exosomes Derived From Exocytosis of Multivesicular Bodies and -Granules

微泡 化学 胞外囊泡 CD63 内体 颗粒(地质) 脂质双层融合 分泌泡 内吞作用
作者
Harry F. G. Heijnen,Anja E. Schiel,Rob Fijnheer,Hans J. Geuze,Jan J. Sixma
出处
期刊:Blood [Elsevier BV]
卷期号:94 (11): 3791-3799 被引量:1271
标识
DOI:10.1182/blood.v94.11.3791
摘要

Abstract Platelet activation leads to secretion of granule contents and to the formation of microvesicles by shedding of membranes from the cell surface. Recently, we have described small internal vesicles in multivesicular bodies (MVBs) and -granules, and suggested that these vesicles are secreted during platelet activation, analogous to the secretion of vesicles termed exosomes by other cell types. In the present study we report that two different types of membrane vesicles are released after stimulation of platelets with thrombin receptor agonist peptide SFLLRN (TRAP) or -thrombin: microvesicles of 100 nm to 1 μm, and exosomes measuring 40 to 100 nm in diameter, similar in size as the internal vesicles in MVBs and -granules. Microvesicles could be detected by flow cytometry but not the exosomes, probably because of the small size of the latter. Western blot analysis showed that isolated exosomes were selectively enriched in the tetraspan protein CD63. Whole-mount immuno-electron microscopy (IEM) confirmed this observation. Membrane proteins such as the integrin chains IIb-β3 and β1, GPIb, and P-selectin were predominantly present on the microvesicles. IEM of platelet aggregates showed CD63+ internal vesicles in fusion profiles of MVBs, and in the extracellular space between platelet extensions. Annexin-V binding was mainly restricted to the microvesicles and to a low extent to exosomes. Binding of factor X and prothrombin was observed to the microvesicles but not to exosomes. These observations and the selective presence of CD63 suggest that released platelet exosomes may have an extracellular function other than the procoagulant activity, attributed to platelet microvesicles.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
子车采蓝发布了新的文献求助10
刚刚
2秒前
万能图书馆应助飘逸宛丝采纳,获得10
3秒前
4秒前
7秒前
清脆如之发布了新的文献求助10
7秒前
戈多发布了新的文献求助10
7秒前
自然映梦发布了新的文献求助10
8秒前
LKSkywalker完成签到,获得积分10
9秒前
9秒前
10秒前
动漫大师发布了新的文献求助10
11秒前
高半青完成签到,获得积分10
11秒前
13秒前
14秒前
squid发布了新的文献求助10
15秒前
酷波er应助时丶倾采纳,获得10
15秒前
斯文败类应助勤恳浩然采纳,获得30
16秒前
16秒前
WYang完成签到,获得积分10
16秒前
17秒前
17秒前
飞飞发布了新的文献求助10
18秒前
活泼纲发布了新的文献求助10
18秒前
18秒前
bkagyin应助33采纳,获得10
19秒前
星辰轨迹完成签到,获得积分10
19秒前
tttt完成签到,获得积分10
20秒前
猪猪包完成签到,获得积分10
21秒前
清脆如之完成签到,获得积分10
21秒前
孤独君浩发布了新的文献求助10
21秒前
刘桔发布了新的文献求助10
23秒前
qqq发布了新的文献求助10
23秒前
25秒前
lwroche完成签到,获得积分10
25秒前
斯文败类应助橙子采纳,获得10
26秒前
26秒前
28秒前
江月年完成签到 ,获得积分10
28秒前
高分求助中
Mass producing individuality 600
Разработка метода ускоренного контроля качества электрохромных устройств 500
Chinesen in Europa – Europäer in China: Journalisten, Spione, Studenten 500
Arthur Ewert: A Life for the Comintern 500
China's Relations With Japan 1945-83: The Role of Liao Chengzhi // Kurt Werner Radtke 500
Two Years in Peking 1965-1966: Book 1: Living and Teaching in Mao's China // Reginald Hunt 500
Epigenetic Drug Discovery 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3823937
求助须知:如何正确求助?哪些是违规求助? 3366251
关于积分的说明 10439654
捐赠科研通 3085409
什么是DOI,文献DOI怎么找? 1697357
邀请新用户注册赠送积分活动 816323
科研通“疑难数据库(出版商)”最低求助积分说明 769569