Olive Oil -Constituents, Quality, Health Properties and Bioconversions 64 techniques, relying on recording of single emission or excitation spectra, are often insufficient if directly applied.In such cases, total luminescence or synchronous scanning fluorescence techniques are used, improving the analytic potential of the fluorescence measurements.With contributions from numerous analytes, the autofluorescence of olive oil exhibits numerous overlapping bands.Such complex spectra should be analyzed using multivariate and multiway methods.Analytical applications of fluorescence to olive oils include discrimination between the different quality grades, adulteration detection, authentication of virgin oils, quantification of fluorescent components, monitoring thermal and photo-oxidation and quality changes during storage.In this chapter the application of fluorescence spectroscopy to qualitative and quantitative analysis of olive oils is reviewed.Methodological aspects of fluorescence measurements and analysis of fluorescence spectra are also discussed. How to referenceIn order to correctly reference this scholarly work, feel free to copy and paste