同位素稀释
化学
色谱法
串联质谱法
氨基酸
质谱法
分析物
生物分析
电喷雾电离
NIST公司
肽
蛋白质质谱法
液相色谱-质谱法
生物化学
自然语言处理
计算机科学
作者
David M. Bunk,Mark S. Lowenthal
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 143-151
被引量:4
标识
DOI:10.1007/978-1-4939-9639-1_12
摘要
The role of amino acid analysis in bioanalysis has changed from a qualitative to a quantitative technique. With the discovery of both electrospray ionization and matrix-assisted laser desorption ionization in the early 1990s, the use of amino acid analysis for qualitative analysis of proteins and peptides has been replaced by mass spectrometry. Accurate measurement of the relative molecular masses of proteins and peptides, peptide mapping, and sequencing by tandem mass spectrometry provide significantly better qualitative information than can be achieved from amino acid analysis. At NIST, amino acid analysis is used to assign concentration values to protein and peptide standard reference materials (SRMs) which, subsequently, will be used in the calibration of a wide variety of protein and peptide assays, such as those used in clinical diagnostics. It is critical that the amino acid analysis method used at NIST for assigning concentration values in SRM deliver the highest accuracy and precision possible. Therefore, we have developed an amino acid analysis method that uses isotope dilution LC-MS/MS-the analytical technique routinely used at NIST to certify analyte concentrations in SRMs for a wide variety of analytes. We present here our most recent method for the quantification of amino acids using isotope dilution LC-MS/MS.
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