Active nanozyme derived from biomineralized metal-organic frameworks for cholesterol detection

Metal-organic framework (MOF) derived nanozymes show significant promises in biosensing and biomedicine. Nanozymes are nanomaterial-based artificial enzymes. But how to improve the catalytic capacity of nanozymes in a simple manner is still a challenge. Herein, we demonstrated that the pore size/volume and catalytic capacity of Mn3O4 can be modulated by biomolecules biomineralized in Mn-MOF before calcination. The biomineralization-derived Mn3O4 shows larger pore size/volume and higher peroxidase/oxidase dual enzymatic activity compared to the pure Mn-MOF derived one. After loading with cholesterol oxidase, the prepared bio-inorganic hybrid nanozyme is able to detect the cholesterol in serum samples with high sensitivity. This work demonstrates that biomineralization of MOF with exogenous molecules is an ingenious and convenient strategy to synthetize nanozyme with high catalytic activity.