原生质体
普氏藻
原丝体
生物
苔藓
再生(生物学)
转染
细胞生物学
植物
基因
遗传学
突变体
作者
Florence Charlot,Guillaume Goudounet,Fabien Nogué,Pierre-François Perroud
出处
期刊:Springer US eBooks
[Springer Nature]
日期:2022-01-01
卷期号:: 3-19
被引量:3
标识
DOI:10.1007/978-1-0716-2164-6_1
摘要
Protoplast production with the moss Physcomitrium (Physcomitrella) patens has a long and successful history. As a tool, it has not only been the base of reverse genetic studies covering research fields as diverse as development, metabolism, or gene network regulation but also allowed its development as a bioengineering platform for protein production. We present here a standardized protocol for protoplast production from Physcomitrium (Physcomitrella) patens protonemata. Additionally, we detail procedures for their transfection, their plating for optimal regeneration, and three alternative selection approaches. To improve the consistency of protoplast regeneration, we describe a new option for protoplast embedding. The use of an alginate matrix to regenerate moss protoplast alleviates the use of warm agarized medium. Thus, it optimizes transformed protoplast survival without any morphological detrimental effect or impact on transfection efficiency.
科研通智能强力驱动
Strongly Powered by AbleSci AI