Role of multistage inoculation on the co-composting of food waste and biogas residue

Effect of diverse Lactobacillus amylophilus, Geobacillus thermoleovorans, and Bacillus subtilis inoculation patterns on the co-composting performance of food waste and biogas residue was explored. Experimental results revealed that, compared to the single-stage inoculation and non-inoculation groups, the multistage inoculation pattern prolonged the thermophilic period during composting, consequently improving organic matter decomposition and humification [with a high germination index (120.9%)]. In addition, it could promote the development of humic substances [with a high humus index (4.3) and biological index (1.4)] and lower emissions of carbon dioxide (CO2), methane (CH4), and ammonia (NH3). Additionally, it could improve the microbial variety and the amounts of functional bacteria (i.e., Chloroflexi) in compost, which might be advantageous for the decomposition of refractory organic materials and plant growth. Therefore, the multistage inoculation pattern is recommended for organic waste composting in terms of its gas emissions, compost quality and efficacy benefits.