Tracking of nascent deoxynucleic acids enable by incorporation of uridine variant with 2 prime azidomethyl tag and click chemistry

We report here the 2′-azidomethyl modified uridine nucleoside (2′-AmU) can be efficiently synthesized and employed in the labeling study of nascent deoxynucleic acid of interest using fluorophore with alkyne (DBCO-Cy5). This new DNA labeling tool using uridine variant (2′-AmU) displays intensive application potential considering the several advantages it possesses, including readily available, rather small size (minimal perturbation), reversible regulation and easy subjected to click reaction.