Agrobacterium-Mediated Transformation of Solanum tuberosum L., Potato

Potato is considered the fourth most important food crop in the world, and the most important non-cereal crop. Potato is transformed using Agrobacterium tumefaciens with relative ease. Several improvements have been made in the last 20 years with respect to tissue culture, transformation, and regeneration of potato. This chapter describes a reliable and efficient potato transformation system using internodal explants. Plasmid preparation, Agrobacterium transformation, potato transformation, regeneration, and recovery are described in detail, as well as molecular characterization of resulting putative transgenic plants.