脂肪生成
脂肪组织
染色
食品科学
组织工程
化学
刺激
生物化学
生物
细胞生物学
内分泌学
遗传学
作者
Frea Mehta,Ruud Theunissen,Mark J. Post
出处
期刊:Methods in molecular biology
日期:2018-10-27
卷期号:: 111-125
被引量:36
标识
DOI:10.1007/978-1-4939-8897-6_8
摘要
Cultured meat, or tissue engineered meat, is a promising alternative to conventional meat production. In order to realistically mimic the multiple tissue types found in beef, food-compatible methods for bovine fat tissue engineering must be developed. We present a protocol for the isolation of adipose tissue-derived preadipocytes and subsequent adipogenic differentiation through free fatty acid stimulation. Differentiating preadipocytes can be either grown in 2D culture conditions or seeded in 3D alginate scaffolds. Differentiation is visually confirmed through lipophilic staining.
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