胞间连丝
韧皮部
维管束
生物物理学
光漂白后的荧光恢复
荧光显微镜
共济体
共焦显微镜
细胞壁
化学
生物
植物
细胞生物学
荧光
质外体
膜
生物化学
物理
量子力学
作者
Helle Juel Martens,Chen Gao,Johannes Liesche
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 215-221
标识
DOI:10.1007/978-1-4939-9562-2_18
摘要
Sugars produced by photosynthesis in leaves get transported to other organs in the phloem vascular tissue. Three general mechanisms have been proposed for the loading of sugars into the phloem. These differ in the involvement of active transport across the phloem cell’s membrane and their capacity for passive intercellular transport through plasmodesmata. This capacity for diffusion from the mesophyll into the phloem cells can be quantified by live-cell microscopy. Instead of sugar molecules, the movement of fluorescent tracers of similar size can be observed. In this chapter, a simple method is described that allows quantification of plasmodesmata-mediated intercellular diffusion across the mesophyll-bundle sheath interface and the bundle sheath-phloem cell interfaces. The fluorescent tracer carboxyfluorescein is loaded into intact leaves and its diffusion monitored with confocal microscopy after photobleaching of a bundle sheath cell.
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