Study on acute toxicity and genotoxicity of Moringa oleifera lam leaves

Objective To evaluate the acute toxicity and genotoxicity of moringa oleifera lam leaves. Ensure safety Moringa oleifera lam leaves, for the Moringa oleifera lam leaves directly as food safety evaluation data provided. Methods The acute toxicity test,Ames test,micronucieus test and sperm shape abnornality test were performed to evaluate the acute toxicity and genotoxicity of Moringa oleifera lam leaves in Hainan. The dose of10.0g/kg bw was chosen for the acute toxicity tests,and the mice were observed for 14 days. The doses of 8,40,200,1 000 and 5 000μg /plate were chosen for the Ames test. The treated plates were cultured at 37 ℃ for 48h,the number of bacterial colony was counted. The doses of 0.83,1.67and 3.33g/ kg were used for the micronucleus test. The number of micronucleus of bone marrow cell was counted,the types and numbers of sperm shape abnormality were assessed. Results The oral MTD of Moringa oleifera lam leaves on male or female mice was over 10.0g/kg.bw in this experiment condition. Ames test,micronecleus test and sperm shape abnormality test were negative. Conclusion Moringa oleifera lam leaves in Hainan was considered non-toxic chemical according to acute toxicity and showed no genotoxicity in this experimental design.