This paper investigates the method of quantitative determination of glutamic acid by colorimetry in the principle of color reaction between α-amino-acids and ninhydrin , and mainly discusses the effect factors , reaction conditions and points for attention of this color reaction . As a method of quantitatively determining glutamic acid ,it is characterized by simplicity and practicability compared with conventional methods of determining glutamic acid . It is available in common lab operations and determination in the process of glutamic acid production.