Studies on chemical constituents in dried buds of Lonicera japonica Thunb.

Objective:To research chemical constituents from the dried buds of Lonicera japonica Thunb.and to find out the active constituents of plant.Methods:The chemical constituents of the plant were identified by means of UV,IR,MS,JH1,D,Z NMR,JC13,D,Z NMR,respectively,in some cases by direct comparison with authentic samples.Study on anti bacterial activity was carried out.Results:Eleven compounds were obtained and identified as luteolin (Ⅰ),corymbosin(Ⅱ),5 hydroxyl 7,3′,4′ trimethoxy flavone(Ⅲ),5 hydroxyl 7,4′ dimethoxy flavone(Ⅳ),quercetin(Ⅴ),lonicerin (Ⅵ), quercetin 3 O β D glucoside(Ⅶ), 3 O [ α L rhamnopyranosyl(1→2) α L arabinopyranosyl] 28 O [ β D glucopyranosyl(1→6) β D glucopyranosyl] oleanolic acid (Ⅷ),chlorogenic acid (Ⅸ),daucosterol (Ⅹ),and sucrose (Ⅺ).Conclusion:Compound Ⅳ was first isolated from Genus Lonicera ,while compounds Ⅴ, Ⅵ, Ⅷ and Ⅹ were isolated from the plant for the first time.Some compounds show anti bacterial activities.