The Subcellular Localizations of Non-structural Proteins of Southern Rice Black-streaked Dwarf Virus

Abstact Studies on the cellular localization patterns of a viral protein can provide clues to understand its mode of action. The six genes encoding non-structural proteins of southern rice black-streaked dwarf virus(SRBSDV) were cloned and expressed in Nicotiana benthamiana cells,respectively. Their subcellular localizations were observed by confocal fluorescent microscopy. The results showed that Pns52 and Pns91 were localized in the cytoplasm and formed large aggregates; Pns6,Pns71,Pns72 and Pns92 were localized in the cell cytoplasm and/or on the cell membrane and all formed reticular strucures attached on cytoskeletons,and Pns6 could also form small aggregates in the cell cytoplasm.