Effects of delayed fluid resuscitation on the inflammatory response in hemorrhagic shock rats

Objective To investigate the effects of delayed fluid resuscitation on the inflammatory response in hemorrhagic shock rats.Methods 40 rats were randomly divided into four lactated Ringer′s solution resuscitation groups(n = 10)including the immediate resuscitation group,the delayed 30 minutes resuscitation group,the delayed 60 minutes resuscitation group,the unresuscitation group.Volume-controlled hemorrhagic shock rat model was made by Wiggers.Their mean arterial pressures were recorded and blood samples were collected at 0,30,90,150,210,270,and 330 minutes after hemorrhage for plasma levels of IL-6,IL-10 and TNF-α.Results Mean blood pressure after immediate resuscitation were not different in the delayed resuscitation groups at any time.TNF-α and IL-6 concentrations of the delayed resuscitation 30 and 60 minutes groups were significantly higher than that of the unresuscitation group(P 0.01),whereas IL-10 concentrations of the delayed resuscitation 60 minutes groups were significantly lower than that of the unresuscitation group(P 0.01).Conclusion Delayed fluid resuscitation induces increase of proinflammatory cytokine production in hemorrhagic shock models,the increase of which is correlated with the time delayed for resuscitation.