Preparation and in vitro evaluation of paclitaxel loaded PLGA nanoparticles by emulsion-solvent evaporation method

Objective To evaluate the paclitaxel(PTX) loaded poly(lactic-co-glycolic acid)(PLGA) nanoparticles prepared by emulsion-solvent evaporation method. Methods The preparation and formulation of the nanoparticles were optimized with single factor tests and an orthogonal design. The PTX-PLGA nanoparticles were characterized with respect to particle size, zeta potential, entrapment efficiency, drug loading, shape, stability and release behavior. Results The mean particle size measured by dynamic light scattering(DLS) was(211.3±1.4) nm, and the zeta potential was(-4.42±0.70) mV. The entrapment efficiency and the drug loading of PTX-NPs were(91.4±3.2)% and(4.35±0.15)%, respectively. The images of TEM show that the nanoparticles were spherical and uniform. In vitro, the PTX-PLGA nanoparticles present good stability and sustained-release behavior. Conclusions The resultant nanoparticles have uniform particle size, high drug loading and good stability. These results are the experimental basis in vitro for the further in vivo pharmacokinetic research of PTX-PLGA nanoparticles.