Laboratory investigation on the influence of one lung ventilation on rabbit lung histomorphology

Objective To investigate on the influence of one lung ventilation(OLV)on rabbit lung histomorphology.Methods Rabbits were randomized into OLV(n=6)and control(n=6)group(two-lung ventrilation).Blood-gas analyses were performed during the mechanical ventilation.All animals were exsanguinated after three hours mechanical ventilation and lungs were excised.The numbers of polymorph nuclear leukocyte(PMN)were counted in lung sections of every rabbits.The ratio of wet lung to dry lung tissue of all animals was calculated.Results Gas exchange function of the non-ventilated lung was impaired after OLV.There were more infiltrations of PMN in the OLV group than in control.The wet/dry lung weight ratio increased significantly in OLV than that in the control.Conclusions OLV can induce acute lung injury more seriously than two-lung ventrilation.