Tyrosine kinase inhibitor A77-1726 inhibiting STAT6 phosphorylation and cfos expression induced by IL-13

Aim To investigate the effects of tyrosine kinase inhibitor A77-1726 on IL-13 induced STAT6 phosphorylation and c-fos expression in Dami cell and to provide novel experimental basis to the clinical application of A77-1726 and the study of IL-13 pathway.Methods Total RNA was extracted from Dami cells incubated with or without IL-13 and A77-1726 respectively for various time points.RT-PCR and agar gel electrophoresis were used to examine the expression of c-fos mRNA.The expression of STAT6 and c-fos proteins was detected by Western blot.A densitometric rel-ative quantitation of PCR and Western blot products was quantitated using Image Quant software.Results STAT6 was phosphorylated by treatment of 100 μg·L-1 IL-13 in Dami cells.Phosphorylation of STAT6 induced by IL-13 was inhibited by treatment of 50μmol·L-1 A77-1726.The expression of c-fos mRNA was significantly induced by IL-13 treatment in Dami cells(P0.05).c-fos mRNA expression induced by IL-13 was inhibited by A77-1726 treatment(P0.05).The expression of c-fos protein was induced by IL-13 treatment in Dami cells(P0.05),and IL-13 induced expression of c-fos was transient.The expression of c-fos protein was also inhibited by A77-1726 treatment(P0.05).Conclusion IL-13 induced phosphorylation of STAT6 and c-fos expression was inhibited by tyrosine kinase inhibitor A77-1726.IL-13 activated JAK/STAT pathway.IL-13 induced c-fos expression was related with STAT6 pathway.