Biomass carbon-based solid acid catalysts are prepared from natural biomass wooden powder using carboniazation-sulphonation method.The effects of the species of wooden powder on catalytic performance of solid acid catalysts are studied.Comparison among carbon-based solid acid catalysts made from different raw materials and their characterization are made.The experimental results show that compared with other solid acid catalysts the carbon-based solid acid catalyst shows higher catalytic performance in esterification.