Distribution of β-Conglycinin in intestines of fishes with different feeding habits

【Objective】 The distribution of β-Conglycinin in intestines of fishes with different feeding habits were investigated in this study.【Method】 Cyprinus carpio(initial weight(31.34±0.29) g),Clarias lazera(initial weight(15.14±0.05) g) and Ctenopharyngodon idellus(initial weight(50.66±0.26) g) were fed on the same semi-purified diets of two isonitrogenous(the mass fraction of crude protein were C.carpio 360.2 g/kg,C.lazera 400.5 g/kg,C.idellus 300.6 g/kg) and isocaloric(C.carpio 15.2 MJ/kg,C.lazera 15.8 MJ/kg,C.idellus 15.6 MJ/kg) formulated with fish meal as control groups,and with purified β-Conglycinin 40 mg/g as experimental groups.The distribution of β-Conglycinin in fishes with different feeding habits were compared using the method of immunohistochemistry after six weeks of feeding trials.【Result】 The intensity optical density(IOD) of β-Conglycinin in the foregut,midgut and hindgut of C.carpio and C.idellus showed an increasing trend,and there were extremely significant(P0.01) differences between the hindgut and foregut of C.carpio,and the IOD of β-Conglycinin in the midgut and hindgut of C.idellus had extremely significant(P0.01) differences,larger than that in the foregut.The IOD of β-Conglycinin in the foregut,midgut and hindgut of C.lazera had no significant(P0.05) difference.【Conclusion】 β-Conglycinin was mainly distributed in the foregut of C.carpio,in the midgut and hindgut of C.idellu and in the foregut,midgut and hindgut of C.lazera,respectively.