A Novel, Helper-Dependent, Adenovirus–Retrovirus Hybrid Vector: Stable Transduction by a Two-Stage Mechanism

Panels B and C of Fig. 3 were mistakenly swapped. Below is how they should have appeared in the May 2002 issue of Molecular Therapy. We apologize for this error. A Novel, Helper-Dependent, Adenovirus–Retrovirus Hybrid Vector: Stable Transduction by a Two-Stage MechanismSoifer et al.Molecular TherapyMay, 2002In BriefWe have developed a novel vector system that uses a helper-dependent adenoviral vector as a carrier to deliver a fully functional retrovirus vector. The helper-dependent adenovirus (HDAd) can accommodate large inserts, provide high titers, and infect nondividing as well as dividing cells. However, adenoviral DNA is rarely integrated into the host cell genome, and its episomal expression is transient. Therefore we inserted a replication-competent, ecotropic retrovirus vector containing the green fluorescent protein (GFP) reporter gene as a second-stage component. Full-Text PDF Open Archive