光漂白
荧光
荧光团
过氧化氢
双光子激发显微术
活性氧
化学
生物物理学
体内
荧光寿命成像显微镜
细胞内
光化学
自体荧光
生物化学
光学
生物
物理
生物技术
作者
Haidong Li,Qichao Yao,Jiangli Fan,Jianjun Du,Jingyun Wang,Xiaojun Peng
标识
DOI:10.1016/j.bios.2017.03.039
摘要
Hydrogen peroxide (H2O2), one of the reactive oxygen species (ROS), plays vital roles in diverse physiological processes. Imbalance of the H2O2 is concerned with serious diseases such as cardiovascular disorders, neurodegenerative diseases, Alzheimer's disease and cancer. Therefore, it is critical to develop efficient methods for monitoring H2O2 in vivo. In this work, a two-photon excitation (860 nm) NIR fluorescent turn-on probe TPNR-H2O2 for H2O2 based on Dicyanomethylene-4H-pyran fluorophore is reported, which can be used in solution detection with 13.2-fold NIR fluorescence enhancement, fast response (completed within 40 min), excellent sensitivity (DL 72.48 nM), and lower cellular auto-fluorescence interference. Importantly, the perfect photostability of TPNR-H2O2 clearly demonstrated that the probe could be applied to imaging intracellular H2O2 for a long time without photobleaching. In addition, through two-photon imaging, this probe was cell permeable and used to monitor the level of endogenous and exogenous H2O2 with promising biological application.
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